answer the question in excel following the orderorder The experiment found the expression of plant-Defence related

answer the question in excel following the order

order

The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants.
Treatment 1negSterile tree
Treatment 2XccThe tree is infected with canker disease in lemon. (Xanthomonas citri pv citri)
Treatment 3PseA plant that has been planted with useful germs (Pseudomonas sp.)
Gene of Interest: Plant-defense related gene
LRRLleucine-rich repeat receptor-like protein gene
RPP13LDisease resistance protein RPP13 gene
CHSTLChalcone synthase Like gene
BSK1LBrassinosteroid-signaling kinase 1 Like gene
House keeping gene
Ef1a gene (Elongation Factor 1 a Gene)
questionFind the expression of Plant-defense related gene (choose 1 gene) method ?
Fold difference = 2^-ΔΔCt
Fill the available values in the table.
Create an easy-to-understand bar chart.
Ready to explain / write a summary
ANSWERFind average cq
Find the SD and consider the statistics to use
Find delta Ct
Find delta delta Ct
Find Fold difference = 2^-ΔΔCt

information

Sample 6 hEf1a gene (House Keeping Gene)RPP13L gene∆Ct∆∆Ct2^-∆∆Ct
replicates for eachcq rep. 1cq rep. 2cq rep. 3ave cqsdcq rep. 1cq rep. 2cq rep. 3ave cqsd
1neg 118.518.5318.4921.6721.521.8
2neg 220.3420.4520.423.323.623.7
3neg 319.0419.1819.1222.4823.2422.96
4neg 420.1320.0520.0622.7322.822.65
5neg 521.7922.3722.0124.8724.5025.02
1xcc 119.3319.3119.322.1322.521.87
2xcc 220.9121.1521.0724.1423.4523.7
3xcc 320.5320.3420.4622.7522.9223.41
4xcc 420.9220.8720.9422.0322.4822.64
5xcc 521.8322.2622.0123.7823.4323.5
1pse 120.9220.6920.7322.4322.8222.23
2pse 219.4219.5019.4721.3821.1121.65
3pse 320.6020.6120.5822.1722.5622.49
4pse 420.0920.5320.4322.0421.5422.16
5pse 523.3123.4323.3525.7625.4024

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