answer the question in excel following the order
order
| The experiment found the expression of plant-Defence related genes in pathogenic infectious conditions and conditions in which beneficial microorganisms coexist with plants. | ||
| Treatment 1 | neg | Sterile tree |
| Treatment 2 | Xcc | The tree is infected with canker disease in lemon. (Xanthomonas citri pv citri) |
| Treatment 3 | Pse | A plant that has been planted with useful germs (Pseudomonas sp.) |
| Gene of Interest: Plant-defense related gene | ||
| LRRL | leucine-rich repeat receptor-like protein gene | |
| RPP13L | Disease resistance protein RPP13 gene | |
| CHSTL | Chalcone synthase Like gene | |
| BSK1L | Brassinosteroid-signaling kinase 1 Like gene | |
| House keeping gene | ||
| Ef1a gene (Elongation Factor 1 a Gene) | ||
| question | Find the expression of Plant-defense related gene (choose 1 gene) method ? | |
| Fold difference = 2^-ΔΔCt | ||
| Fill the available values in the table. | ||
| Create an easy-to-understand bar chart. | ||
| Ready to explain / write a summary | ||
| ANSWER | Find average cq | |
| Find the SD and consider the statistics to use | ||
| Find delta Ct | ||
| Find delta delta Ct | ||
| Find Fold difference = 2^-ΔΔCt | ||
information
| Sample 6 h | Ef1a gene (House Keeping Gene) | RPP13L gene | ∆Ct | ∆∆Ct | 2^-∆∆Ct | |||||||||||
| replicates for each | cq rep. 1 | cq rep. 2 | cq rep. 3 | ave cq | sd | cq rep. 1 | cq rep. 2 | cq rep. 3 | ave cq | sd | ||||||
| 1 | neg 1 | 18.5 | 18.53 | 18.49 | 21.67 | 21.5 | 21.8 | |||||||||
| 2 | neg 2 | 20.34 | 20.45 | 20.4 | 23.3 | 23.6 | 23.7 | |||||||||
| 3 | neg 3 | 19.04 | 19.18 | 19.12 | 22.48 | 23.24 | 22.96 | |||||||||
| 4 | neg 4 | 20.13 | 20.05 | 20.06 | 22.73 | 22.8 | 22.65 | |||||||||
| 5 | neg 5 | 21.79 | 22.37 | 22.01 | 24.87 | 24.50 | 25.02 | |||||||||
| 1 | xcc 1 | 19.33 | 19.31 | 19.3 | 22.13 | 22.5 | 21.87 | |||||||||
| 2 | xcc 2 | 20.91 | 21.15 | 21.07 | 24.14 | 23.45 | 23.7 | |||||||||
| 3 | xcc 3 | 20.53 | 20.34 | 20.46 | 22.75 | 22.92 | 23.41 | |||||||||
| 4 | xcc 4 | 20.92 | 20.87 | 20.94 | 22.03 | 22.48 | 22.64 | |||||||||
| 5 | xcc 5 | 21.83 | 22.26 | 22.01 | 23.78 | 23.43 | 23.5 | |||||||||
| 1 | pse 1 | 20.92 | 20.69 | 20.73 | 22.43 | 22.82 | 22.23 | |||||||||
| 2 | pse 2 | 19.42 | 19.50 | 19.47 | 21.38 | 21.11 | 21.65 | |||||||||
| 3 | pse 3 | 20.60 | 20.61 | 20.58 | 22.17 | 22.56 | 22.49 | |||||||||
| 4 | pse 4 | 20.09 | 20.53 | 20.43 | 22.04 | 21.54 | 22.16 | |||||||||
| 5 | pse 5 | 23.31 | 23.43 | 23.35 | 25.76 | 25.40 | 24 | |||||||||
