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Biology Lab Activities: Cellular Respiration
Background Questions
Based on what you’ve learned in class, in your textbook, and from using Visible Biology, answer the following questions about cellular respiration.
1. Fill in the blanks in the cellular respiration equation: C6H12O6 + 6 _______ → 6 CO2 + 6 ______ + up to 38 molecules of _______.
2. What are the reactants involved in cellular respiration?
3. What are the products of cellular respiration?
4. Cellular respiration consists of four main phases. Complete the following sentences about these phases and where they take place.
a. ______ takes place in the cytosol.
b. Pyruvate oxidation takes place in the ______.
c. The ______ cycle takes place in the mitochondrial matrix.
d. Oxidative phosphorylation takes place in the _______________.
5. In 2–3 sentences, explain why cellular respiration is important for a eukaryote’s survival.
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Lab 1: Mitochondria Structures, Reactants, and Products of Cellular Respiration
Activity 1: Label a mitochondrion
1. Launch the view
· Launch Visible Biology.
· Navigate to Study/Lab Activities, and find the Cellular Respiration Lab section.
· Select view 1. Mitochondrion (Cellular Respiration).
2. Label the image below
· Explore the 3D model of the mitochondrion going through cellular respiration to find the structures you need to label.
· Fill in the blanks to label the structures from the list below.
Part A: Label the mitochondria structures
Word List:
1
Cristae
Inner membrane
Matrix
Outer membrane
___
___
___
___
Part B: Label the reactants and products of glycolysis
Word List:
ATP
ADP
Glucose (C6H12O6) molecule
Pyruvates
___
___
___
___
Part C: Label the reactants and products of pyruvate oxidation and the citric acid cycle
Word List:
Acetyl-CoA
ATP
Carbon dioxide (CO2) molecules
___
___
___
Part D: Label the reactants and products of oxidative phosphorylation
Word List:
ATP
Oxygen (O2) molecules
Water (H2O) molecules
___
___
___
Name: Date:
Lab 1: Mitochondria Structures, Reactants, and Products of Cellular Respiration
Activity 2: Explore the roles mitochondria structures play in cellular respiration
Refer to your labeled mitochondrion model from Activity 1 and the content in Visible Biology. Based on what you’ve learned, match each of the following structures with the brief description of its role in cellular respiration.
Structures:
a. Inner membrane
b. Matrix
c. Cristae
d. Cytosol
Descriptions:
___ This structure contains the electron transport chain, and chemiosmosis occurs across this structure to produce water molecules and ATP.
___ These structures increase the surface area of the inner mitochondrial membrane to facilitate gas exchange.
___ This fluid surrounds the mitochondria, and it’s where glycolysis takes place.
___ This is the fluid-filled inner part of the mitochondrion, where the citric acid cycle takes place.
Name: Date:
Lab 1: Mitochondria Structures, Reactants, and Products of Cellular Respiration
Activity 3: Explore the reactants and products of cellular respiration
Refer to your labeled reactants and products of cellular respiration from Activity 1 and the content in Visible Biology. Based on what you’ve learned, complete the following table on the molecules involved in cellular respiration.
Molecule Type |
Reactant/Product |
Where It Comes From |
Stage(s) It’s Part of |
Its Significance |
Carbon Dioxide | Product | Made in the ____________ | Pyruvate oxidation and citric acid cycle | It is a byproduct of cellular respiration essential for ____________. |
Oxygen | Reactant | From __________from the lungs or the _____ surrounding the cell | Oxidative phosphorylation | It combines with ___________ to form water molecules during oxidative phosphorylation. |
Glucose | Reactant | From ___________ an animal/human consumes or produced by plants via ___________ | Glycolysis | It is converted into two __________ during glycolysis. |
Water | Product | Made in the _____________ | Oxidative phosphorylation | It is a byproduct of cellular respiration essential for ______________. |
ATP | Product | Made in the cytosol and the mitochondrial _______ and inner membrane | Glycolysis, citric acid cycle, and oxidative phosphorylation | It provides the energy that powers many ________________. |
Based on what you’ve learned from labeling the mitochondrion (cellular respiration) model and completing the table, fill in the blanks to balance the cellular respiration equation:
C6H12O6 + 6 _______ → 6 ________ + 6 H2O + up to 38 molecules of _________.
Name: Date:
Lab 2: Cellular Respiration in the Mitochondrion
Refer to your labeled reactants and products of cellular respiration image from Lab 1, Activity 1.
1. In the space that follows, draw a mitochondrion (or paste a screenshot of your drawing). Your drawing should include the following:
a. Inside the mitochondrion, draw the inner and outer membranes, cristae, and matrix.
b. Outside the mitochondrion, draw the cytosol, as well as the ATP molecules, carbon dioxide (CO2) molecules, water (H2O) molecules, oxygen (O2) molecules, glucose (C6H12O6) molecules, NAD+, NADH, FAD, FADH2, and electrons with arrows showing how they move into, out of, or through the mitochondrion. Be sure to accurately portray the number of each of these molecules in your drawing.
2. Based on your drawing and what you’ve learned about cellular respiration, put the following steps of cellular respiration into the correct order (from 1–5).
___ Within the mitochondrion, the pyruvates are converted into acetyl-CoA.
___ Glucose is converted into fructose, which is converted into two pyruvates.
___ The electron transport chain and chemiosmosis produce water molecules and ATP.
___ Acetyl-CoA goes into the citric acid cycle, producing carbon dioxide, ATP, NADH, FADH2, and electrons.
___ NADH and FADH2 bring electrons to the electron transport chain.
3. In your own words, briefly describe the cellular respiration equation.
4. The equation for photosynthesis is 6CO2 + 6H2O → C6H12O2 + 6O2. Based on this equation and what you’ve learned about cellular respiration, how do these two processes relate to each other?
